An example of a lineweaver burk plot in biochemistry the lineweaver burk plot or double reciprocal plot is a graphical representation of the lineweaver burk equation of enzyme kinetics described by hans lineweaver and dean burk in 1934. A quick guide for my students on how to use excel to get km and vmax. Enzymatic inhibition and lineweaver burk plots enzymatic inhibition and lineweaver burk plots so the first thing that im going to talk about is something called a lineweaver burke plot and how it allows us to look at the michaelis menten equation in a different way so what im going to do is take the michaelis mentin equation but . A plot of 1 v 0 versus 1 s called a lineweaver burk or double reciprocal plot yields a straight line with an intercept of 1 v max and a slope of k m v max figure 836 the intercept on the x axis is 1 k m . The lineweaver burk plot was widely used to determine important terms in enzyme kinetics such as km and vmax before the wide availability of powerful computers and non linear regression software
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